association between twenty five gens with stroke subtypes in a persian population, a database structure

stroke is the third cause of death in the world after the cancer and cardiovascular disease. . it has been shown that stroke in northeast of iran occurs approximately one decade earlier than in western countries. reasons for the high stroke incidence among persian are not yet clear. it is the first gene-association study in persian population, which determines the relationship between 25 gene variations and stroke. this genes are involved in 3 cellular pathways and in single nucleotide polymorphisms (snps) in mirna genes. so we made a genetic stroke databases with more than 1000 stroke patients for this region. 25 gene polymorphisms were analyzed and registries in this database. furthermore this database is part of international stroke genetics consortium . in the present study aimed to determine if there is any association between 22 genetic variations in protein-coding genes and also 11 polymorphisms in mirna-coding regions. in this case-control study patients with ischemic stroke and subjects without any history of vascular diseases were recruited. all cases were diagnosed via computed tomography (ct) scans and magnetic resonance imaging (mri).  genotyping was gen in cases and controls using real-time, pcr-rflp and multiplex arms-pcr method. statistical analyses were performed using spss v.21 software and a pvalue< 0.05 was considered significant. this investigation indicates there may be significant association between variations in 14 of 32 polymorphism and ischemic stroke in population of northeastern iran (p-value<0.05).  the present study reveals some of the potential genetic risk factors that may contribute to ischemic stroke. additional analysis and genome-wide techniques bring better understanding of the genetic background of this complex disorder.